董小鸥

董 小 鸥

教授,博士生导师

地址:江苏省南京市玄武区卫岗1号,南京农业大学第三实验楼2期309

邮箱:odong@njau.edu.cn


研究兴趣

1. 作物基因编辑新技术的开发与优化

2. 应用基因编辑技术创制突破性水稻种质


招聘博士后1名,依托课题组申报南京农业大学钟山青年研究院岗位,待遇从优,并赋予独立承担主要课题的机会。


教育与工作经历

2020.11 -  至今  bwin体育必赢|首頁-Welcome!    教授


2016.9 -2020.9  美国加州大学戴维斯分校(UC Davis)植物病理系    博士后


2010.9 - 2016.8  加拿大英属哥伦比亚大学(UBC)植物系    硕博连读(植物学)


2010.6 - 2010.8  北京生命科学研究所(NIBS)    实习科研助理


2006.9 - 2010.6  香港大学    学士(生物技术)


代表性研究成果

建立了将有益基因定向插入在“基因组安全港”(Genomic Safe Harbors)的育种策略,在不改变作物现有优异性状的同时引入新的性状。利用CRISPR/Cas基因编辑技术,将不含标记基因的胡萝卜素合成相关DNA大片段(5.2 kb)精确定点插入到水稻基因组安全港位点,获得了性状优良,且富含重要营养物质beta胡萝卜素的水稻植株。

阐明了一系列植物NLR免疫蛋白的协同工作机制。通过遗传学和生物化学的手段,在模式植物拟南芥中揭示了NLR免疫蛋白SNC1及其辅助NLR蛋白家族SIKIC的协同工作机理,并发现两条泛素化通路分别对SNC1及SIKIC进行调控。此外,通过对于辅助NLR蛋白家族ADR1的研究,揭示了其成员间的复杂调控机理,同时阐述了ADR1蛋白家族在各类免疫蛋白通路中的作用。这些研究深化了我们对于植物免疫机理的认知。


发表论文

1.    Dong OX and Ronald PC (2021). Targeted gene insertion in plants. PNAS. doi:10.1073/pnas.2004834117.

2.    Dong OX (2020). Gene replacement and knockin made easy in plants. Molecular Plant. 13(8):1104.

3.    McWhite CD, Papoulas O, Drew K, Cox RM, June V, Dong OX, Kwon T, Wan C, Salmi ML, Roux SJ, Browning KS, Chen ZJ, Ronald PC, Marcotte EM (2020). A pan-plant protein complex map reveals deep conservation and novel assemblies. Cell. 181(2):460-474.

4.    Dong OX, Shu Y, Jain R, Zhang N, Duong P, Butler C, Li Y, Lipzen A, Martin J, Barry J, Schmutz J, Tian L, and Ronald PC (2020). Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9. Nature Communications. 11(1):1-10.

5.    Dong OX and Ronald PC (2020). Fast-track for engineered plants. Nature Biotechnology. 38(1):32-34.

6.    Dong OX and Ronald PC (2019). Genetic engineering for disease resistance in plants: recent progress and future perspectives. Plant Physiology. 180(1):26-38.

7.    Li Y, Cao XL, Zhu Y, Yang XM, Zhang KN, Xiao ZY, Wang H, Zhao JH, Zhang LL, Li GB, Zheng YP, Fan J, Wang J, Chen XQ, Wu XJ, Zhao JQ, Dong OX, Chen XW, Chern M, and Wang WM (2019). Osa-miR398b boosts H2O2 production and rice blast disease-resistance via multiple superoxide dismutases. New Phytologist. 222(3):1507-1522.

8.    Wu Z, Li M, Dong OX, Xia S, Liang W, Bao Y, Wasteneys G, and Li X (2019). Differential regulation of TNL-mediated immune signaling by redundant helper CNLs. New Phytologist. 222(2):938-953.

9.    Dong OX#, Ao K#, Xu F#, Johnson K, Wu Y, Li L, Xia S, Liu Y, Huang Y, Xia S, Rodriguez E, Chen X, Chen S, Zhang Y, Petersen M, and Li X (2018). Individual components of paired typical NLR immune receptors are regulated by distinct E3 ligases. Nature Plants. 4(9):699-710.

10.  Dong OX#, Tong M#, Bonardi V, Kasmi EK, Woloshen V, Wunsch LK, Dangl JL, and Li X (2016). TNL-mediated immunity in Arabidopsis requires complex regulation of the redundant ADR1 family. New Phytologist. 210(3):960-73.

11.  Dong OX, Meteignier LV, Plourde MB, Ahmed B, Wang M, Jensen C, Jin H, Moffett P, Li X, and Germain H (2016). Arabidopsis TAF15b localizes to RNA processing bodies and contributes to snc1-mediated autoimmunity. Molecular Plant-Microbe Interactions. 29(4):247-57.

12.  Huang S, Monaghan J, Zhong X, Lin L, Sun T, Dong OX, and Li X (2014). HSP90s are required for NLR immune receptor accumulation in Arabidopsis. The Plant Journal. 79(3):427-439.

13.  Johnson K, Dong OX, Huang Y, and Li X (2013). A Rolling Stone Gathers No Moss, but Resistant Plants Must Gather Their MOSes. Cold Spring Harbour Symposia on Quantitative Biology. 77: 259-68.

14.  Liu J, Ding P, Sun T, Nitta Y, Dong OX, Huang X, Yang W, Li X, Botella JR, and Zhang Y (2013). Heterotrimeric g proteins serve as a converging point in plant defense signaling activated by multiple receptor-like kinases. Plant Physiology. 161(4): 2146-58.

15.  Kong Q, Qu N, Gao M, Zhang Z, Ding X, Yang F, Li Y, Dong OX, Chen S, Li X, and Zhang Y (2012). The MEKK1-MKK1/MKK2-MPK4 kinase cascade negatively regulates immunity mediated by a mitogen-activated protein kinase kinase kinase in Arabidopsis. Plant Cell. 24(5): 2225-36.

16.  Johnson K, Dong OX, and Li X (2011). The evolutionarily conserved MOS4-associated complex. Central European Journal of Biology. 6(5): 776-784.

17.  Li Y, Dong OX, Johnson K, and Zhang Y (2011). MOS1 epigenetically regulates the expression of plant Resistance gene SNC1. Plant Signal Behav. 6(3): 434-6.

18.  Dong OX, Johnson K, Li X, and Zhang Y (2011). The mRNA export pathway in plants. Frontiers in Biology. 6(3): 246–250.

19.  Germain H, Qu N, Cheng Y, Lee E, Huang Y, Dong OX, Gannon P, Huang S, Ding P, Li Y, Sack F, Zhang Y, and Li X (2010). MOS11: a new component in the mRNA export pathway. PLOS Genetics. 6(12): e1001250.



科研项目

南京农业大学高层次引进人才启动资金    2020-2025    150万    主持    


其他学术活动

Molecular Plant 专题编辑  (Feature Editor)

Frontiers in Plant Science 评审编辑 (Review Editor)

Nature Communications, Molecular Plant-Microbe Interactions, Plant Methods, Plant Communications, Frontiers in Plant Science等杂志审稿人

南京农业大学本科生课程《基因工程导论》客座讲师(2020)

加州大学戴维斯分校本科生课程 Genetics and Society 客座讲师 (2019及2020)

美国自然科学基金NSF科普活动主讲(2018)

博士生期间担任本科生课程Molecular Genetics,Basic Genetics,Plant Physiology助教(2010-2012)